Clarification of Cell Culture Broth to recover secreted products (e.g. antibodies, AAV vectors, etc.)
Single Use BioSettler is useful as a selective cell retention device for perfusion bioreactor cultures of CHO cells
Cell Therapy Applications
Harvesting, Washing and Concentration of Cell Therapy Products (e.g. CAR-T, β islet cells, etc.)
Inclined Settling Bioreactor
Cell culture parameters (pH, dissolved oxygen, and temperature) measured with fluorescent dye sensors inserted from the top of BioSettler and controlled by sparged gas mix of air, oxygen and carbon dioxide manipulated by automation and control system.
In Situ Product Recovery
Oil water emulsion, created in the bioreactor to extract toxic metabolite product, is pumped into an external BioSettler, where it is rapidly separated into an oil phase leaving at the top and the water phase being recycled from the bottom of BioSettler to the bioreactor.
Sudhin BioSettler's many applications
Single Use Disposable BioSettler has been demonstrated as a selective cell retention device for CHO perfusion bioreactors to achieve and maintain high cell densities and high viabilities.
Sudhin BioSettler is being demonstrated for clarification of cell culture broth to recover secreted products (e.g. antibodies, AAV vectors, etc.). Video on the right shows cell clumps settling down fast in real time.
Sudhin BioSettler is being tested for separating β islet cells from single iPSCs for the treatment of type1 diabetes.
Sudhin BioSettler can be used for Harvesting, Washing and Concentration of Cell Therapy Products (e.g. CAR-T cells).